Gene search

Sequence information

Select Gene Cds Cds_length GC_content Pep Pep_length
Bda09g01152 ATGAAGGAGTCAAAGTCTGAGTTTATGGTGAAGGAAATTCAGACGGAGCGAAAAGGCAGACGGAGTGGACAGCTAGACGGAGTGAAAATGGAGAGTCTGTGTTTAGAAGAAAGAAATCTGGAGTATCGTTCATGTGCAGGATGGAACAGTTTATCAAGGTACAGCACAGAGAGAGAGGAATACTTCATCCAGACGCAGCGTATAAGTCCAGACGTTGGGGATGACGTCCGCGCTTGGTCTGTTCTTCCTCCAGACGTAGTGTTTAACTCCAGAGTGTGGGACGACGTGAGGGTTAGGAGGGCGGAAGAGCATTTGCTGTATCTGGAATTGCACCTTCCAGTCAGCGGCTGGAAATGGATCCAGCGACGGAATCCAGCGTCTAAGTTTCCGACGGATTTCGGATTAGTTTCGATCCAAGGCTCTGTGAGAGAGAAGTTGATCAACGAGCTTCAACGTTTGGAGCTGGTGAAAGATGTGAATGTGGATGCGGTATATAAAAGAGTTCTGCTTAAAGAGGAGAGGCGCAGGGTTGGAGCTTTTAGATCTCAAGTTACTTCATTGTTTGGAGCTGATAACTTGTGGGGAAAAGGTTATACTGGTTCCAAAGTTAAAATGGCTATTTTTGACACTGGTATTCGAGCCAATCATCCACATTTTCGTAATATCAAGGAGCGAACCAATTGGACAAACGAGGATACATTAAATGACAATCTTGGGCATGGAACATTTGTCGCTGGTGTTATTGCAGGTGTAGATGCTGAATGTCTCGGATTTGCTCCAGATACAGAGATTTATGCTTTTCGTGTTTTTACAGATGCACAGGTATCTTATACTTCATGGTTCCTTGACGCATTCAATTATGCCATGTCTACCAATATAGATGTACTTAATTTAAGCATTGGTGGACCAGATTACTTGGATCTTCCTTTTGTAGAGAAGATTTGGGAAATAACAGCTAATAACATCATCATGGTGTCTGCAATTGGAAACGATGGACCACTTTATGGCACTTTAAACAATCCTGCTGATCAAAGTGATGTTATTGGTGTTGGTGGTATTGACTACAGTGATCATATTGCTTCATTTTCATCTCGTGGAATGAGTACATGGGAGCTACCTCATGGTTATGGTCGTGTTAAGCCAGATGTTGTTGCCTATGGACGGGATATTATGGGATCCAAGATTAGTACTGGTTGCAAAAGTTTATCAGGCACTAGTGTTGCAAGCCCTGTGGTTGCTGGTGTGGTGTGTCTACTTGTGAGTGTCATTTCTGAAAAACATCGAAAGGATGTTTTGAACCCAGCTAGCATGAAGCAAGCACTTGTTGAAGGGGCTGCGAAGCTTTCTGGTCCTAACATGTATGAACAGGGTGCTGGGAGAGTTGATCTGTTAGAATCTTTTGAAATCCTTCAGAATTACCAACCCCGTGCCAGCATATTCCCTAGTGTTCTTGACTACACGGATTGCCCTTACAGCTGGCCTTTTTGTCGTCAACCACTTTACGCAGGTGCCATGCCTGTTATCTTTAATGCTACCATTTTGAATGGACTGGGCGTAATCGGTTATGTTGAAAGTCCACCAACGTGGCATCCATTTGATGATGAAGGCAATCTTCTAAGTATCCACTTCACTTATTCAGAAGTCATCTGGCCTTGGACTGGCTATATAGCACTACATATGCAAATTAAGGAAGAAGGAGCCCAGTTTTCTGGAGAGATTGAGGGAAACGTGACTTTAAAGGTTCATAGTCCCCCGGGAAAAGGAGAGAAGCACCAAAGGATAAGTACCTGTGTTCTTCAATTGAAGCTAAAAGTTGTTCCAACGCCAGCAAGATCAAGACGGGTTTTGTGGGATCAGTTTCACAGTATTAAGTATCCTCCTGGTTACATTCCGAGAGACTCATTGGACGTTCGTAATGACATACTTGATTGGCATGGGGACCATTTACATACAAACTTTCATATCATGTTTAACAATCTAAGGGATGCTGGATACTTTATTGAGACTCTTGGATCTCCTTTTACATGTTTTGATGCTAGCCAATATGGAACTCTCTTGTTAGTGGATCTTGAGGATGAGTTCTTTCAAGAAGAGATTGAAAAGTTAAGGGACGATGTTTTAAACAATGGATTAGGGTTGGCTGTGTTTGCTGAATGGTATAATGTGGAAACCATGGTGAAGATGAGATTCTTTGATGACAATACAAGGAGCTGGTGGACTCCCGTTACTGGAGGTGCTAATGTCCCAGCACTAAATAATCTCTTGGCTCCATTTGGTATTGCTTTTGGTGATAAGATTCTAAATGGCGATTTTTCTATTGATGGTGAGCAGAGTAGATATGCATCTGGGACAGATATTGTAAGGTTTCCCTTGGGTGGTTACATGCACAGCTTCCCCTTCCTGGATAGTTCAGAAAGTGGGGCCACTCAGAACGTTCTGACATCTGGCATGAGTAGAGATGACTCCCCTATTCTTGGACTTTTGGAGTCGGGGGAAGGTCGAATTGCTGTATATGGGGATTCCAACTGTTTGGACAGCAGCCATATGGTTACCAATTGCTACTGGCTCTTGGGGAAAATATTAGATTTCACGAGTGGAAATATCAAAGATCCAGTGCTTTTCTCAGACTCCGCTAAACAAGATTCACCCTTGCATCAAGAAGACAACCAGTTACCATCACGCAGGACTGATGTTAATTTCTCTGCATATTCTGCCGTTGTTGGAAAGGAATTAATCTGCAGAAGTGACTCTAGATTTGAAATTTGGGGCACAAAAGGATATAGCAGTTTGCAAGGTAGGGGAAGGAACAGAAGATTGCCAGGTTTTCCTGTGGTCGACTTGAAAAGAGGTTCAAATTCTACAACAGAAGTTTCTGTGTTGAGGTTACCCAATGTTAAGAGCAAAAATGATTCTGTTGGAAACAATTACCTGAGCCTTTTCCATCCGGATGAGCCTGACGTGCTTATGCTTGGTTCTAGTCATTGGCTTGTGCCTGCAGTAGTTGCAATTACCGGTTAG 3018 43.07 MKESKSEFMVKEIQTERKGRRSGQLDGVKMESLCLEERNLEYRSCAGWNSLSRYSTEREEYFIQTQRISPDVGDDVRAWSVLPPDVVFNSRVWDDVRVRRAEEHLLYLELHLPVSGWKWIQRRNPASKFPTDFGLVSIQGSVREKLINELQRLELVKDVNVDAVYKRVLLKEERRRVGAFRSQVTSLFGADNLWGKGYTGSKVKMAIFDTGIRANHPHFRNIKERTNWTNEDTLNDNLGHGTFVAGVIAGVDAECLGFAPDTEIYAFRVFTDAQVSYTSWFLDAFNYAMSTNIDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGGIDYSDHIASFSSRGMSTWELPHGYGRVKPDVVAYGRDIMGSKISTGCKSLSGTSVASPVVAGVVCLLVSVISEKHRKDVLNPASMKQALVEGAAKLSGPNMYEQGAGRVDLLESFEILQNYQPRASIFPSVLDYTDCPYSWPFCRQPLYAGAMPVIFNATILNGLGVIGYVESPPTWHPFDDEGNLLSIHFTYSEVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLKVHSPPGKGEKHQRISTCVLQLKLKVVPTPARSRRVLWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHLHTNFHIMFNNLRDAGYFIETLGSPFTCFDASQYGTLLLVDLEDEFFQEEIEKLRDDVLNNGLGLAVFAEWYNVETMVKMRFFDDNTRSWWTPVTGGANVPALNNLLAPFGIAFGDKILNGDFSIDGEQSRYASGTDIVRFPLGGYMHSFPFLDSSESGATQNVLTSGMSRDDSPILGLLESGEGRIAVYGDSNCLDSSHMVTNCYWLLGKILDFTSGNIKDPVLFSDSAKQDSPLHQEDNQLPSRRTDVNFSAYSAVVGKELICRSDSRFEIWGTKGYSSLQGRGRNRRLPGFPVVDLKRGSNSTTEVSVLRLPNVKSKNDSVGNNYLSLFHPDEPDVLMLGSSHWLVPAVVAITG 1005
       

Gff information

Chromosome Start End Strand Old_gene Gene Num
9 42263620 42278838 + Bda034139 Bda09g01152 13402

Annotation

Select Seq ID Length Analysis Description Start End IPR GO
Bda09g01152 1005 ProSitePatterns Serine proteases, subtilase family, serine active site. 404 414 IPR023828 -
Bda09g01152 1005 ProSitePatterns Serine proteases, subtilase family, histidine active site. 240 250 IPR022398 -
Bda09g01152 1005 CDD Peptidases_S8_SKI-1_like 194 450 IPR034185 -
Bda09g01152 1005 Gene3D Peptidase S8/S53 domain 170 466 IPR036852 GO:0004252|GO:0006508
Bda09g01152 1005 SUPERFAMILY Subtilisin-like 142 520 IPR036852 GO:0004252|GO:0006508
Bda09g01152 1005 PANTHER MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-1 PROTEASE 101 866 - -
Bda09g01152 1005 Pfam Subtilase family 200 450 IPR000209 GO:0006508|GO:0008236
Bda09g01152 1005 PANTHER PEPTIDASE S8 101 866 - -
Bda09g01152 1005 ProSiteProfiles Serine proteases, subtilase domain profile. 181 470 - -
Bda09g01152 1005 PRINTS Subtilisin serine protease family (S8) signature 200 219 IPR015500 -
Bda09g01152 1005 PRINTS Subtilisin serine protease family (S8) signature 403 419 IPR015500 -
Bda09g01152 1005 PRINTS Subtilisin serine protease family (S8) signature 236 249 IPR015500 -
       

Pathway

Select Query KO Definition Second KO KEGG Genes ID GHOSTX Score
Bda09g01152 K08653 MBTPS1; membrane-bound transcription factor site-1 protease [EC:3.4.21.112] - csv:101222509 1570.44
       

Dupl-types

Select Gene1 Location1 Gene2 Location2 E-value Duplicated-type
Bda09g01152 Bda-Chr9:42263620 Bda09g00075 Bda-Chr9:1422336 2.32E-08 dispersed
Bda09g01152 Bda-Chr9:42263620 Bda10g00887 Bda-Chr10:43336039 2.76E-07 transposed
       

Deco-Alignment

Select Vvi1 Blo1 Blo2 Bda1 Bda2 Bpe1 Bpe2 Bma1 Bma2 Cmo1 Cmo2 Cma1 Cma2 Car1 Car2 Sed1 Cpe1 Cpe2 Bhi1 Tan1 Cmetu1 Lac1 Hepe1 Mch1 Lcy1 Cla1 Cam1 Cec1 Cco1 Clacu1 Cmu1 Cre1 Cone1 Cone2 Cone3 Cone4 Lsi1 Csa1 Chy1 Cme1 Blo3 Blo4 Bda3 Bda4 Bpe3 Bpe4 Bma3 Bma4 Sed2 Cmo3 Cmo4 Cma3 Cma4 Car3 Car4 Cpe3 Cpe4 Bhi2 Tan2 Cmetu2 Lac2 Hepe2 Mch2 Lcy2 Cla2 Cam2 Cec2 Cco2 Clacu2 Cmu2 Cre2 Lsi2 Csa2 Chy2 Cme2
Vvi11g337 . . . . . . Bma07g00493 . Cmo10g00658 . . . . . Sed12g2432 . Cpe18g00430 Bhi02g01135 Tan09g1284 Cmetu02g0878 . Hepe09g0762 . . . . . . . . . Cone2ag0295 Cone16ag0742 . . Lsi06g00869 . . Cme02g01368 Blo08g00292 . . Bda09g01152 Bpe08g00475 . . . . . . Cma10g00622 . Car10g00574 . . . . . . . . . . Cla06g00853 Cam06g0921 Cec06g0929 Cco06g0956 Clacu06g0922 Cmu06g0889 Cre06g1670 . Csa01g01728 Chy02g01868 .
       

Syn-Families

Select Gene Event_type S_start S_end Function Ath_gene Identity(%) E-value Score
Bda12g00712 . 1 767 Subtilisin-like Serine Gene Family AT1G04110 67.4 3.7e-311 1063.9
Bda01g00472 . 5 729 Subtilisin-like Serine Gene Family AT1G20150 52.8 3.8e-207 718.4
Bda01g00472 . 2 475 Subtilisin-like Serine Gene Family AT1G20160 56.6 4.8e-140 495.0
Bda07g00866 . 15 765 Subtilisin-like Serine Gene Family AT1G30600 67.8 6.1e-304 1040.0
Bda06g01090 . 52 769 Subtilisin-like Serine Gene Family AT1G32940 55.3 1.2e-226 783.1
Bda06g01090 . 52 769 Subtilisin-like Serine Gene Family AT1G32950 54.3 9.5e-221 763.5
Bda06g01090 . 25 769 Subtilisin-like Serine Gene Family AT1G32960 55.6 1.6e-237 819.3
Bda06g01090 . 24 769 Subtilisin-like Serine Gene Family AT1G32970 50.5 2.9e-201 698.7
Bda01g01763 . 36 771 Subtilisin-like Serine Gene Family AT2G04160 56.3 3.3e-243 838.2
Bda07g00820 . 3 644 Subtilisin-like Serine Gene Family AT2G04160 59.5 2.3e-225 778.9
Bda02g00365 . 23 761 Subtilisin-like Serine Gene Family AT2G05920 69.9 1.2e-306 1048.9
Bda02g00348 . 23 761 Subtilisin-like Serine Gene Family AT2G05920 69.9 1.6e-306 1048.5
Bda01g01993 . 19 757 Subtilisin-like Serine Gene Family AT2G05920 68.2 1.1e-288 989.2
Bda11g01464 . 26 761 Subtilisin-like Serine Gene Family AT2G05920 56.6 1.6e-231 799.3
Bda08g01361 . 25 760 Subtilisin-like Serine Gene Family AT2G05920 54.5 6.5e-220 760.8
Bda07g01841 . 27 778 Subtilisin-like Serine Gene Family AT2G05920 50.4 1.3e-212 736.5
Bda05g00622 . 27 777 Subtilisin-like Serine Gene Family AT2G05920 51.5 7.2e-203 704.1
Bda01g01594 . 35 764 Subtilisin-like Serine Gene Family AT2G05920 52.4 2.1e-202 702.6
Bda10g00887 . 25 776 Subtilisin-like Serine Gene Family AT2G05920 51.0 8.2e-199 690.6
Bda13g00001 . 6 618 Subtilisin-like Serine Gene Family AT2G05920 51.3 1.3e-175 613.6
Bda10g00876 . 1 522 Subtilisin-like Serine Gene Family AT2G05920 55.9 5.8e-160 561.6
Bda01g01898 . 20 819 Subtilisin-like Serine Gene Family AT2G19170 82.3 0.0e+00 1349.7
Bda11g00106 . 6 819 Subtilisin-like Serine Gene Family AT2G19170 81.5 0.0e+00 1349.0
Bda11g01464 . 7 761 Subtilisin-like Serine Gene Family AT3G14067 53.0 1.4e-222 769.6
Bda02g00348 . 27 761 Subtilisin-like Serine Gene Family AT3G14067 53.6 1.6e-218 756.1
Bda02g00365 . 27 761 Subtilisin-like Serine Gene Family AT3G14067 53.6 2.1e-218 755.7
Bda10g00876 . 1 529 Subtilisin-like Serine Gene Family AT3G14067 69.0 6.0e-213 737.6
Bda01g01993 . 21 755 Subtilisin-like Serine Gene Family AT3G14067 54.0 6.7e-212 734.2
Bda08g01361 . 27 760 Subtilisin-like Serine Gene Family AT3G14067 51.8 6.9e-209 724.2
Bda10g00887 . 1 782 Subtilisin-like Serine Gene Family AT3G14067 51.3 4.5e-200 694.9
Bda05g00622 . 24 783 Subtilisin-like Serine Gene Family AT3G14067 50.7 3.6e-197 685.3
Bda13g00001 . 3 617 Subtilisin-like Serine Gene Family AT3G14067 51.3 1.0e-164 577.4
Bda10g00887 . 24 784 Subtilisin-like Serine Gene Family AT3G14240 76.2 0.0e+00 1180.2
Bda05g00622 . 33 785 Subtilisin-like Serine Gene Family AT3G14240 74.3 0.0e+00 1147.1
Bda11g01464 . 28 757 Subtilisin-like Serine Gene Family AT3G14240 53.2 6.6e-220 760.8
Bda02g00365 . 27 757 Subtilisin-like Serine Gene Family AT3G14240 50.9 2.8e-210 728.8
Bda02g00348 . 27 757 Subtilisin-like Serine Gene Family AT3G14240 50.7 1.4e-209 726.5
Bda01g01993 . 22 751 Subtilisin-like Serine Gene Family AT3G14240 51.7 1.5e-208 723.0
Bda08g01361 . 28 756 Subtilisin-like Serine Gene Family AT3G14240 51.1 2.2e-207 719.2
Bda07g01841 . 31 782 Subtilisin-like Serine Gene Family AT3G14240 50.2 2.9e-207 718.8
Bda01g01594 . 38 760 Subtilisin-like Serine Gene Family AT3G14240 50.5 1.1e-203 706.8
Bda13g00001 . 3 618 Subtilisin-like Serine Gene Family AT3G14240 59.2 1.3e-202 703.4
Bda10g00876 . 1 520 Subtilisin-like Serine Gene Family AT3G14240 51.9 3.7e-146 515.8
Bda06g00103 . 2 444 Subtilisin-like Serine Gene Family AT4G00230 50.7 1.7e-138 490.0
Bda09g00063 . 11 391 Subtilisin-like Serine Gene Family AT4G00230 50.3 1.5e-94 344.0
Bda06g01090 . 52 769 Subtilisin-like Serine Gene Family AT4G10510 54.3 2.7e-223 771.9
Bda06g01090 . 22 769 Subtilisin-like Serine Gene Family AT4G10520 54.9 4.8e-231 797.7
Bda06g01090 . 22 769 Subtilisin-like Serine Gene Family AT4G10530 52.9 3.2e-219 758.4
Bda06g01090 . 24 769 Subtilisin-like Serine Gene Family AT4G10540 54.1 2.8e-234 808.5
Bda06g01090 . 52 769 Subtilisin-like Serine Gene Family AT4G10550 53.3 6.6e-222 767.3
Bda07g00866 . 13 765 Subtilisin-like Serine Gene Family AT4G20430 71.8 0.0e+00 1102.0
Bda01g01751 . 1 285 Subtilisin-like Serine Gene Family AT4G21320 75.9 9.6e-132 466.5
Bda06g01090 . 26 764 Subtilisin-like Serine Gene Family AT4G21630 52.1 1.2e-205 713.4
Bda06g01090 . 26 764 Subtilisin-like Serine Gene Family AT4G21650 52.1 9.5e-211 730.3
Bda01g01898 . 7 819 Subtilisin-like Serine Gene Family AT4G30020 81.2 0.0e+00 1352.4
Bda11g00106 . 6 819 Subtilisin-like Serine Gene Family AT4G30020 80.9 0.0e+00 1345.9
Bda13g00001 . 1 623 Subtilisin-like Serine Gene Family AT4G34980 74.4 1.3e-276 949.1
Bda05g00622 . 28 785 Subtilisin-like Serine Gene Family AT4G34980 54.3 4.2e-227 784.6
Bda10g00887 . 14 784 Subtilisin-like Serine Gene Family AT4G34980 52.8 9.4e-227 783.5
Bda11g01464 . 22 757 Subtilisin-like Serine Gene Family AT4G34980 51.1 3.7e-207 718.4
Bda02g00348 . 25 757 Subtilisin-like Serine Gene Family AT4G34980 50.1 2.1e-202 702.6
Bda02g00365 . 25 757 Subtilisin-like Serine Gene Family AT4G34980 50.1 2.8e-202 702.2
Bda10g00876 . 1 520 Subtilisin-like Serine Gene Family AT4G34980 53.2 6.0e-149 525.0
Bda09g00063 . 28 390 Subtilisin-like Serine Gene Family AT5G03620 53.5 4.5e-104 375.6
Bda09g01152 . 274 1005 Subtilisin-like Serine Gene Family AT5G19660 79.6 0.0e+00 1223.4
Bda07g00866 . 12 541 Subtilisin-like Serine Gene Family AT5G44530 72.3 9.1e-236 813.1
Bda09g00832 . 1 627 Subtilisin-like Serine Gene Family AT5G45640 51.9 1.6e-173 606.7
Bda09g00832 . 1 629 Subtilisin-like Serine Gene Family AT5G45650 57.7 8.9e-204 707.2
Bda07g01841 . 23 782 Subtilisin-like Serine Gene Family AT5G51750 69.9 0.0e+00 1105.1
Bda11g01464 . 26 761 Subtilisin-like Serine Gene Family AT5G51750 52.7 7.6e-224 773.9
Bda08g01361 . 9 760 Subtilisin-like Serine Gene Family AT5G51750 51.3 2.7e-221 765.4
Bda02g00348 . 23 761 Subtilisin-like Serine Gene Family AT5G51750 50.3 2.4e-217 752.3
Bda02g00365 . 23 761 Subtilisin-like Serine Gene Family AT5G51750 50.3 3.1e-217 751.9
Bda13g00001 . 3 623 Subtilisin-like Serine Gene Family AT5G51750 50.9 5.0e-175 611.7
Bda09g00063 . 25 562 Subtilisin-like Serine Gene Family AT5G59190 50.2 6.5e-142 501.5
Bda01g01763 . 23 774 Subtilisin-like Serine Gene Family AT5G59810 63.6 1.8e-281 965.3
Bda07g00820 . 1 647 Subtilisin-like Serine Gene Family AT5G59810 68.0 8.3e-263 903.3
Bda11g01464 . 7 762 Subtilisin-like Serine Gene Family AT5G67360 71.9 0.0e+00 1097.0
Bda08g01361 . 10 761 Subtilisin-like Serine Gene Family AT5G67360 66.7 9.8e-293 1002.7
Bda01g01594 . 17 765 Subtilisin-like Serine Gene Family AT5G67360 61.8 5.4e-267 917.1
Bda02g00365 . 15 762 Subtilisin-like Serine Gene Family AT5G67360 56.2 1.7e-228 789.3
Bda02g00348 . 15 762 Subtilisin-like Serine Gene Family AT5G67360 56.2 2.2e-228 788.9
Bda07g01841 . 25 778 Subtilisin-like Serine Gene Family AT5G67360 54.7 2.9e-228 788.5
Bda01g01993 . 22 755 Subtilisin-like Serine Gene Family AT5G67360 56.8 7.9e-226 780.4
Bda10g00887 . 18 779 Subtilisin-like Serine Gene Family AT5G67360 51.4 4.4e-208 721.5
Bda05g00622 . 25 780 Subtilisin-like Serine Gene Family AT5G67360 50.3 1.1e-203 706.8
Bda14g01267 . 1 527 Subtilisin-like Serine Gene Family AT5G67360 53.4 1.3e-199 693.3
Bda13g00001 . 4 618 Subtilisin-like Serine Gene Family AT5G67360 54.7 7.0e-182 634.4
Bda10g00876 . 1 522 Subtilisin-like Serine Gene Family AT5G67360 53.7 1.2e-149 527.3
       

Syn-Orthogroups

Select Orthogroup Bda Bhi Blo Bma Bpe Cam Car Cco Cec Chy Cla Clacu Cma Cme Cmetu Cmo Cmu Cone Cpe Cre Csa HCH Hepe Lac Lcy Lsi Mch Sed Tan Vvi Total
OG0010952 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 1 31
       

Transcriptome

Select Gene Chr Type da1 da2 da3 da4 da5 da6 da7 da8 da9 da10
Bda09g01152 Bda_Chr09 FPKM 1.635143 1.656237 0.0 0.0 0.0 0.0 0.0 0.0 0.0